肾移植术后早期的高尿酸血症与结石形成（附四例报告）

本文报告了４例肾移植患者术后１－３个月出现高尿酸血症并形成了移植肾、输尿管尿酸盐结石，结合复习文献探讨了环孢素Ａ（ＣｓＡ）引起高尿酸血症的可能机制和促成结石形成的因素。通过回顾４例患者成功治疗的体验，讨论了移植肾、输尿管尿酸结石预防及治疗的选择。     

斑点免疫渗滤法检测丙肝IgG抗体试剂盒的研制

用基因工程重组的丙肝抗原包被于硝酸纤维素膜，建立了检测丙肝ＩｇＧ抗体的斑点免疫渗滤法。与ＥＬＩＳＡ试剂盒进行双盲式同步测定，二法检验结果差异无显著性。渗滤法简便快速，适用于各级医院，有很强的推广价值。     

反义脱氧寡核苷酸对SRS病毒复制的抑制作用

用合成的反义脱氧寡核苷酸,包括修饰的和非修饰的,同聚的和异聚的脱氧寡核苷酸对SRS病毒感染细胞的增殖、细胞群落形成,XC融合细胞产生的抑制作用。结果表明,上述的寡聚物对病毒都有抑制活性。各种寡聚物的作用机制不同。     

时滞系统鲁棒稳定性的新判据

基于Ｌｙａｐｎｕｏｖ泛函方法，研究了满足匹配条件的不确定性时滞系统的鲁棒稳定性及具有稳定度β〉０鲁棒稳定性，给出了已有结果不能推出的新判据。这些新判据对已有结果难以使用的情况下也可有是有效的，或有时减少了已有结果的保守性。     

人胎儿胃液、胆汁及胃粘膜、胆囊组织胃动素水平及其相关性

作者采用特异性胃动素(MTL)抗体,通过放射免疫法,对8例16～40周龄的人胎儿胃液、胆汁及胃粘膜、胆囊组织MTL含量进行探测。结果显示:MTL含量胃液为46.00±12.87pmol/L,胆汁为29.89±15.45Pmol/L;胃粘膜为1.34±0.45pmol/g,胆囊为0.87±0.32pmol/g.胃液、胃粘膜MTL含量分别高于胆汁及胆囊MTL水平(P<0.05)。相关分析显示:胃液与胃粘膜MTL含量,胆汁与胆囊MTL含量均呈显著正相关(N=8,r=0.8933,P<0.01;N=8,r=0.9016,P<0.01)。胃粘膜MTL含量与胎龄增长也呈显著正相关(N=8,r=0.7321,P<0.05)。     

胰岛素对数种缩血管物质作用的影响

目的 观察胰岛素对去甲肾上腺素、内皮素-１、氯化钾（ＫＣＩ）、血管紧张素Ⅱ和５－羟色胺缩血管作用的影响。方法 用ＳＤ大鼠的离体动脉血管观察缩血管物质的作用。结果 在含有１ｍＵ／ｍｌ胰岛素环境中，胰岛素对数种缩血管作用机制不同的物质有程度不同的血管增敏作用。对去甲肾上腺素、内皮素－１和ＫＣＩ有明显的增敏效果；对血管紧张素Ⅱ有增敏趋势，但无显著性差异；对5－羟色胺无增敏效应。结论 胰岛素在高血压的发病过程中可能起重要作用。     

胎儿腹壁异常的超声诊断

本文总结了胎儿腹壁异常１２例，其中脐疝４例，腹壁缺损４例、膈疝３例、胸腹壁缺损１例。分析了超声图像特点及漏诊、误诊原因，并提出鉴别诊断要点及最早诊断时间。对分娩方式及时间选择有重要意义。     

荧光定量聚合酶链反应在尖锐湿疣诊断中的应用

目的　探讨荧光定量聚合酶链反应 (FQ - PCR)对尖锐湿疣 (CA )的诊断价值。方法　 FQ- PCR检测病理确诊的 CA患者 36份标本和健康人 84份标本 ;FQ- PCR及病理诊断同期检测临床送检病例 2 73份标本 ,并做诊断性试验评价。结果　 36例病理确诊的 CA患者 HPV6和 HPV11的 DNA FQ- PCR全部阳性 ,平均拷贝数为 1.0× 10 7± 1.0× 10 2 / m l;84例健康人 FQ- PCR全部阴性 ,平均拷贝数为 3× 10± 2× 10 / ml。 FQ- PCR与病理诊断符合率为 10 0 % ,CA患者与健康人拷贝数的差异有显著性 (P<0 .0 5 )。FQ- PCR与病理诊断对比得出 :灵敏度为 10 0 % ,特异度为 92 % ,误诊率为 0 .0 8,漏诊率为 0 ,准确度为 98.9% ,阳性预测值为 98.8% ,阴性预测值为 10 0 % ,阳性似然比为 12 .5 ,阴性似然比为 0。结论　 FQ - PCR能准确定量 ,灵敏度高 ,特异性强 ,快速 ,简便 ,可作为尖锐湿疣早期诊断的指标     

Neurons labeled from locomotor-related ventrolateral funiculus stimulus sites in the neonatal rat spinal cord.

Spinal cord/brainstem preparations from 5- to 8-day-old rats, maintained in vitro, were used to determine the cells of origin and regions of termination of fibers in the superficial ventrolateral funiculus (VLF) at a site from which rhythmic locomotor-like activity can be induced. Rhythmic locomotor-like activity was recorded from lumbar ventral roots after short trains of stimuli (50 Hz for 0.5-2 seconds) delivered to the VLF. Field potential mapping revealed that single VLF stimuli elicited responses in the ipsilateral ventrolateral medulla. Tract-tracing experiments by using biocytin, pressure-injected into the VLF, showed that only a small number of brainstem neurons were labeled and these were scattered bilaterally in the ventrolateral and lateral medulla. Dense concentrations of nerve terminals were found in the lateral reticular nucleus ipsilateral to the stimulation site. Labeled spinal cord neurons included a primary population of large cells distributed bilaterally in lamina VII from T13 to L4, with peak numbers in L2 ipsilaterally and in L3 contralaterally. Intracellular recordings revealed that some L2 and L3 neurons with rhythmic responses to VLF stimulation could be activated antidromically from the VLF, with latencies of less than 1.0 msec. These observations led us to speculate that the superficial VLF carries a locomotor-related tract originating bilaterally in lumbar lamina VII and terminating in the ipsilateral medulla, including the lateral reticular nucleus. This pathway may be part of the spinoreticular or spinoreticulotectal pathway that has been described in many species, the function of which has only loosely been ascribed.